docs/cookbook/YEp24PGK_XK.gb
LOCUS name 11452 bp DNA circular UNK 01-JAN-1980
DEFINITION description.
ACCESSION id
VERSION id
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin 530..1690
/note="2 micron origin"
gene 2507..3310
/gene="URA3"
CDS 2507..3310
/gene="URA3"
/codon_start=1
/transl_table=11
/product="URA3"
/protein_id="AGE61868.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
regulatory 3452..4934
/regulatory_class="promoter"
/note="from Saccharomyces cerevisiae PGK1"
primer_bind 4953..4967
/label="primer1"
/PCR_conditions="primer
sequence:GCGGATCCTCTAGAATGGTTTGTTCAGTAATTCAG"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
primer_bind complement(6729..6749)
/label="primer3"
/PCR_conditions="primer
sequence:AGATCTGGATCCTTAGATGAGAGTCTTTTCCAG"
/ApEinfo_fwdcolor="#baffa3"
/ApEinfo_revcolor="#ffbaba"
regulatory 6756..7120
/regulatory_class="terminator"
/note="from Saccharomyces cerevisiae PGK1"
gene 7178..8368
/gene="tet"
CDS 7178..8368
/gene="tet"
/note="pBR322 marker"
/codon_start=1
/transl_table=11
/product="tetracyclin resistance protein"
/protein_id="AGE61866.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
gene complement(10387..11247)
/gene="bla"
CDS complement(10387..11247)
/gene="bla"
/note="ampicillin resistance protein"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AGE61867.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
61 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagcta gctttgaaga
121 aaaatgcgcc ttattcaatc tttgctataa aaaatggccc aaaatctcac attggaagac
181 atttgatgac ctcatttctt tcaatgaagg gcctaacgga gttgactaat gttgtgggaa
241 attggagcga taagcgtgct tctgccgtgg ccaggacaac gtatactcat cagataacag
301 caatacctga tcactacttc gcactagttt ctcggtacta tgcatatgat ccaatatcaa
361 aggaaatgat agcattgaag gatgagacta atccaattga ggagtggcag catatagaac
421 agctaaaggg tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac
481 aggaggtact agactacctt tcatcctaca taaatagacg catataagta cgcatttaag
541 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata
601 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc
661 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gaaagtatag
721 gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa
781 aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg
841 ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc
901 acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct
961 agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac
1021 aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt
1081 tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg
1141 cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc
1201 gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag
1261 aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat
1321 caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta
1381 gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt
1441 tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
1501 gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc
1561 ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat
1621 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag
1681 atgcttcgtt aacaaagata tgctattgaa gtgcaagatg gaaacgcaga aaatgaaccg
1741 gggatgcgac gtgcaagatt acctatgcaa tagatgcaat agtttctcca ggaaccgaaa
1801 tacatacatt gtcttccgta aagcgctaga ctatatatta ttatacaggt tcaaatatac
1861 tatctgtttc agggaaaact cccaggttcg gatgttcaaa attcaatgat gggtaacaag
1921 tacgatcgta aatctgtaaa acagtttgtc ggatattagg ctgtatctcc tcaaagcgta
1981 ttcgaatatc attgagaagc tgcagcgtca catcggataa taatgatggc agccattgta
2041 gaagtgcctt ttgcatttct agtctctttc tcggtctagc tagttttact acatcgcgaa
2101 gatagaatct tagatcacac tgcctttgct gagctggatc aatagagtaa caaaagagtg
2161 gtaaggcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt agacggagta
2221 tactagtata gtctatagtc cgtggaattc tcatgtttga cagcttatca tcgataagct
2281 agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc ggtttctttg
2341 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
2401 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
2461 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
2521 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
2581 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
2641 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
2701 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
2761 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
2821 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
2881 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
2941 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac
3001 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
3061 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
3121 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
3181 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
3241 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
3301 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
3361 ttcaatttaa ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga
3421 cgaaaaaaaa aaaattggaa agaaaaagct ttctaactga tctatccaaa actgaaaatt
3481 acattcttga ttaggtttat cacaggcaaa tgtaatttgt ggtattttgc cgttcaaaat
3541 ctgtagaatt ttctcattgg tcacattaca acctgaaaat actttatcta caatcatacc
3601 attcttataa catgtcccct taatactagg atcaggcatg aacgcatcac agacaaaatc
3661 ttcttgacaa acgtcacaat tgatccctcc ccatccgtta tcacaatgac aggtgtcatt
3721 ttgtgctctt atgggacgat ccttattacc gctttcatcc ggtgatagac cgccacagag
3781 gggcagagag caatcatcac ctgcaaaccc ttctatacac tcacatctac cagtgtacga
3841 attgcattca gaaaactgtt tgcattcaaa aataggtagc atacaattaa aacatggcgg
3901 gcacgtatca ttgcccttat cttgtgcagt tagacgcgaa tttttcgaag aagtaccttc
3961 aaagaatggg gtctcatctt gttttgcaag taccactgag caggataata atagaaatga
4021 taatatacta tagtagagat aacgtcgatg acttcccata ctgtaattgc ttttagttgt
4081 gtatttttag tgtgcaagtt tctgtaaatc gattaatttt tttttctttc ctctttttat
4141 taaccttaat ttttatttta gattcctgac ttcaactcaa gacgcacaga tattataaca
4201 tctgcacaat aggcatttgc aagaattact cgtgagtaag gaaagagtga ggaactatcg
4261 catacctgca tttaaagatg ccgatttggg cgcgaatcct ttattttggc ttcaccctca
4321 tactattatc agggccagaa aaaggaagtg tttccctcct tcttgaattg atgttaccct
4381 cataaagcac gtggcctctt atcgagaaag aaattaccgt cgctcgtgat ttgtttgcaa
4441 aaagaacaaa actgaaaaaa cccagacacg ctcgacttcc tgtcttccta ttgattgcag
4501 cttccaattt cgtcacacaa caaggtccta gcgacggctc acaggttttg taacaagcaa
4561 tcgaaggttc tggaatggcg ggaaagggtt tagtaccaca tgctatgatg cccactgtga
4621 tctccagagc aaagttcgtt cgatcgtact gttactctct ctctttcaaa cagaattgtc
4681 cgaatcgtgt gacaacaaca gcctgttctc acacactctt ttcttctaac caagggggtg
4741 gtttagttta gtagaacctc gtgaaactta catttacata tatataaact tgcataaatt
4801 ggtcaatgca agaaatacat atttggtctt ttctaattcg tagtttttca agttcttaga
4861 tgctttcttt ttctcttttt tacagatcat caaggaagta attatctact ttttacaaca
4921 aatataaaac caaaagatcc tctagaatgg tttgttcagt aattcagaga cagacaagag
4981 aggtttccaa cacaatgtct ttagactcat actatcttgg gtttgatctt tcgacccaac
5041 aactgaaatg tctcgccatt aaccaggacc taaaaattgt ccattcagaa acagtggaat
5101 ttgaaaagga tcttccgcat tatcacacaa agaagggtgt ctatatacac ggcgacacta
5161 tcgaatgtcc cgtagccatg tggttagagg ctctagatct ggttctctcg aaatatcgcg
5221 aggctaaatt tccattgaac aaagttatgg ccgtctcagg gtcctgccag cagcacgggt
5281 ctgtctactg gtcctcccaa gccgaatctc tgttagagca attgaataag aaaccggaaa
5341 aagatttatt gcactacgtg agctctgtag catttgcaag gcaaaccgcc cccaattggc
5401 aagaccacag tactgcaaag caatgtcaag agtttgaaga gtgcataggt gggcctgaaa
5461 aaatggctca attaacaggg tccagagccc attttagatt tactggtcct caaattctga
5521 aaattgcaca attagaacca gaagcttacg aaaaaacaaa gaccatttct ttagtgtcta
5581 attttttgac ttctatctta gtgggccatc ttgttgaatt agaggaggca gatgcctgtg
5641 gtatgaacct ttatgatata cgtgaaagaa aattcagtga tgagctacta catctaattg
5701 atagttcttc taaggataaa actatcagac aaaaattaat gagagcaccc atgaaaaatt
5761 tgatagcggg taccatctgt aaatatttta ttgagaagta cggtttcaat acaaactgca
5821 aggtctctcc catgactggg gataatttag ccactatatg ttctttaccc ctgcggaaga
5881 atgacgttct cgtttcccta ggaacaagta ctacagttct tctggtcacc gataagtatc
5941 acccctctcc gaactatcat cttttcattc atccaactct gccaaaccat tatatgggta
6001 tgatttgtta ttgtaatggt tctttggcaa gggagaggat aagagacgag ttaaacaaag
6061 aacgggaaaa taattatgag aagactaacg attggactct ttttaatcaa gctgtgctag
6121 atgactcaga aagtagtgaa aatgaattag gtgtatattt tcctctgggg gagatcgttc
6181 ctagcgtaaa agccataaac aaaagggtta tcttcaatcc aaaaacgggt atgattgaaa
6241 gagaggtggc caagttcaaa gacaagaggc acgatgccaa aaatattgta gaatcacagg
6301 ctttaagttg cagggtaaga atatctcccc tgctttcgga ttcaaacgca agctcacaac
6361 agagactgaa cgaagataca atcgtgaagt ttgattacga tgaatctccg ctgcgggact
6421 acctaaataa aaggccagaa aggacttttt ttgtaggtgg ggcttctaaa aacgatgcta
6481 ttgtgaagaa gtttgctcaa gtcattggtg ctacaaaggg taattttagg ctagaaacac
6541 caaactcatg tgcccttggt ggttgttata aggccatgtg gtcattgtta tatgactcta
6601 ataaaattgc agttcctttt gataaatttc tgaatgacaa ttttccatgg catgtaatgg
6661 aaagcatatc cgatgtggat aatgaaaatt gggatcgcta taattccaag attgtcccct
6721 taagcgaact ggaaaagact ctcatctaag gatctcccat gtctctactg gtggtggtgc
6781 ttctttggaa ttattggaag gtaaggaatt gccaggtgtt gctttcttat ccgaaaagaa
6841 ataaattgaa ttgaattgaa atcgatagat caattttttt cttttctctt tccccatcct
6901 ttacgctaaa ataatagttt attttatttt ttgaatattt tttatttata tacgtatata
6961 tagactatta tttatctttt aatgattatt aagattttta ttaaaaaaaa attcgctcct
7021 cttttaatgc ctttatgcag tttttttttc ccattcgata tttctatgtt cgggttcagc
7081 gtattttaag tttaataact cgaaaattct gcgttcgtta aagctttaat gcggtagttt
7141 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
7201 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
7261 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
7321 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
7381 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
7441 gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat
7501 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
7561 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
7621 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
7681 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
7741 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
7801 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
7861 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
7921 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
7981 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
8041 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
8101 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
8161 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
8221 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
8281 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
8341 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
8401 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
8461 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
8521 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
8581 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
8641 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
8701 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
8761 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
8821 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
8881 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
8941 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga
9001 ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
9061 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
9121 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
9181 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
9241 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
9301 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
9361 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
9421 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
9481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
9541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
9601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
9661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
9721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
9781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
9841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
9901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
9961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
10021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
10081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
10141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
10201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
10261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
10321 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
10381 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
10441 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
10501 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
10561 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
10621 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
10681 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
10741 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
10801 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
10861 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
10921 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
10981 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
11041 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
11101 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
11161 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
11221 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
11281 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
11341 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
11401 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aa
//