docs/cookbook/pGREG505.gb
LOCUS pGREG505 9465 bp ds-DNA circular 15-DEC-2012
DEFINITION pGREG505 circular
ACCESSION pGREG505
VERSION pGREG505
KEYWORDS .
SOURCE .
ORGANISM . .
COMMENT
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
primer_bind 9444..9463
/label=T3
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
primer_bind complement(3597..3614)
/label=M13-fwd
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
primer_bind 9406..9426
/label=M13-rev
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
primer_bind complement(3568..3588)
/label=T7
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
rep_origin 8356..9038
/label=ColE1 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
rep_origin 3775..4081
/label=F1 ori
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
rep_origin 3758..4213
/label=M13 origin
/ApEinfo_fwdcolor=gray50
/ApEinfo_revcolor=gray50
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
CDS 3685..3753
/label=LacZ alpha
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_binding 9378..9400
/label=LacO
/ApEinfo_fwdcolor=#6495ed
/ApEinfo_revcolor=#6495ed
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_recomb 1997..2030
/label=LoxP
/ApEinfo_fwdcolor=steel blue
/ApEinfo_revcolor=orange
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_recomb 3499..3532
/label=LoxP(1)
/ApEinfo_label=LoxP
/ApEinfo_fwdcolor=steel blue
/ApEinfo_revcolor=orange
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
CDS 7599..8261
/label=AmpR
/ApEinfo_fwdcolor=yellow
/ApEinfo_revcolor=yellow
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 40..483
/label=GAL1p
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 530..562
/label=rec1
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 1725..1759
/label=rec2
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
misc_feature 565..1724
/label=HIS3
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
width 5 offset 0
ORIGIN
1 aaaagctgga gctcgtttaa acggcgcgcc tagtacggat tagaagccgc cgagcgggtg
61 acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg
121 aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
181 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac
241 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
301 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg
361 cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg
421 taataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
481 aaaccccgga ttctagagcg gccgcactag tggatccccc gggctgcagg aattcgatat
541 caagcttatc gataccgtcg acgtcatatg gatccgctgc acggtcctgt tccctagcat
601 gtacgtgagc gtatttcctt ttaaaccacg acgctttgtc ttcattcaac gtttcccatt
661 gtttttttct actattgctt tgctgtggga aaaacttatc gaaagatgac gactttttct
721 taattctcgt tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca
781 cggcattagt cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact
841 cttggcctcc tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt
901 tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga
961 attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc
1021 aagataaacg aaggcaaaga tgacagagca gaaagcccta gtaaagcgta ttacaaatga
1081 aaccaagatt cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat
1141 cttcccagaa aaagaggcag aagcagtagc agaacaggcc acacaatcgc aagtgattaa
1201 cgtccacaca ggtatagggt ttctggacca tatgatacat gctctggcca agcattccgg
1261 ctggtcgcta atcgttgagt gcattggtga cttacacata gacgaccatc acaccactga
1321 agactgcggg attgctctcg gtcaagcttt taaagaggcc ctactggcgc gtggagtaaa
1381 aaggtttgga tcaggatttg cgcctttgga tgaggcactt tccagagcgg tggtagatct
1441 ttcgaacagg ccgtacgcag ttgtcgaact tggtttgcaa agggagaaag taggagatct
1501 ctcttgcgag atgatcccgc attttcttga aagctttgca gaggctagca gaattaccct
1561 ccacgttgat tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc
1621 tcttgcggtt gccataagag aagccacctc gcccaatggt accaacgatg ttccctccac
1681 caaaggtgtt cttatgtagt gacaccgatt atttaaagct gcaggtcgac ctcgagtcat
1741 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
1801 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
1861 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
1921 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
1981 ttgcggccgg tacctaataa cttcgtatag catacattat acgaagttat attaagggtt
2041 ctcgacgttt tcgacactgg atggcggcgt tagtatcgaa tcgacagcag tatagcgacc
2101 agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg
2161 cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta aaatagaaca
2221 actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat ctttttattg
2281 tcagtactga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
2341 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
2401 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
2461 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
2521 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
2581 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
2641 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
2701 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
2761 aatcaggata ttcttctaat acctggaatg ctgttttgcc ggggatcgca gtggtgagta
2821 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
2881 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
2941 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
3001 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
3061 ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat ggttgtttat gttcggatgt
3121 gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag
3181 tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc
3241 gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct
3301 tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg
3361 ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag
3421 gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagcta
3481 aacagatctc tagacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg
3541 ttgtcttaat taagggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
3601 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
3661 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
3721 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
3781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
3841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
3901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
3961 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
4021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
4081 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
4141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
4201 acgtttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4261 gcatatcgac ggtcgaggag aacttctagt atatccacat acctaatatt attgccttat
4321 taaaaatgga atcccaacaa ttacatcaaa atccacattc tcttcaaaat caattgtcct
4381 gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt atactctatt
4441 tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc aagaaatatc
4501 ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt tcgaatctct
4561 tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct atcgcacaga
4621 atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct
4681 ttttcaactg aaaaattggg agaaaaagga aaggtgagag gccggaaccg gcttttcata
4741 tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat
4801 tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt
4861 ttcttacctt ttacatttca gcaatatata tatatatttc aaggatatac cattctaatg
4921 tctgccccta tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa
4981 gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc
5041 aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgtccca
5101 cttccagatg aggcgctgga agcctccaag aaggttgatg ccgttttgtt aggtgctgtg
5161 ggtggtccta aatggggtac cggtagtgtt agacctgaac aaggtttact aaaaatccgt
5221 aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta
5281 gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa
5341 ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg
5401 gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg
5461 gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc
5521 tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt ccctacattg
5581 aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac
5641 ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga tgaagcctcc
5701 gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag
5761 aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat
5821 aaggttgacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac
5881 ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc
5941 agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa
6001 gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacataaac
6061 tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat atgttcatag
6121 ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa aaaggaggat
6181 agtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
6241 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
6301 agaaaatcat gaaactacga ttcctaattt gatattggag gattttctct aaaaaaaaaa
6361 aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac
6421 cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa
6481 cggccttacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata
6541 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
6601 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
6661 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
6721 ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta acttacacgc
6781 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta
6841 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga
6901 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt
6961 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa
7021 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa
7081 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc
7141 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt
7201 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg
7261 aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
7321 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
7381 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
7441 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
7501 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
7561 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
7621 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
7681 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
7741 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
7801 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
7861 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
7921 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
7981 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
8041 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
8101 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
8161 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
8221 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
8281 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
8341 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
8401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
8461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
8521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
8581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
8641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
8701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
8761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
8821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
8881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
8941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
9001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
9061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
9121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
9181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
9241 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
9301 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
9361 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
9421 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaac
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