recordings/Acceptance-staff_admin-provider_521206357/visiting-provider-TIB-dois_3257149311/recording.har
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Als Mitglieder der nikotinischen Azetylcholinrezeptor-Familie (nAChR) besitzen der GlyR und der GABAAR einen pentameren Aufbau. Beim GlyR sind vier α-Untereinheiten (α1-α4 UE) und eine β-UE bekannt die jeweils aus einer extrazellulären N-terminalen Domäne, 4 Transmembrandomänen und einer großen intrazellulären Schleife bestehen. Die GABAARs können sich aus einer Vielzahl von 21 UEs α(1-6), β(1-3), γ(1-3), δ, ε, π, θ, ρ(1-3) zusammensetzen. Eine hohe lokale Konzentration von GlyRs und GABAARs an inhibitorischen Synapsen ist entscheidend für eine effiziente glyzin-/GABAerge Signal-Transmission im Nervensystem und wird durch die Interaktion mit dem Verankerungprotein Gepyhrin gewährleistet. Aufgrund der Dimerisierungs – und Trimerisierungseigenschaften der Gephyrin - Domänen wird angenommen, dass sich unterhalb der postsynaptischen Membran eine hexagonale Matrix ausbildet. In der hexagonalen Matrix entstehen hochaffine Bindestellen für die ß-UE des GlyR und die β-UEs und α-UEs des GABAAR. Die Gephyrin – Bindemotive (gbm) sind in der intrazellulären Schleife (TM3-TM4) der Untereinheiten zu finden. Bei der Akkumulierung von Gephyrin unterhalb der postsynaptischen Membran nimmt der Gehirn-spezifische GDP/GTP Austausch Faktor Collybistin (Cb) eine tragende Rolle ein. So wird angenommen das Cb über die Interaktion der Pleckstrin-homologen Domäne (PH) mit membranständigen PI3P Gephyrin-CbII Komplexe zur postsynaptischen Membran rekrutiert. Ebenfalls eine wichtige Funktion bei der Rekrutierung des Geph-Cb - Komplexes zur Membran nimmt die src homologe 3 (SH3) – Domäne ein. Die SH3 – Domäne agiert als auto – inhibitorische Domäne und belässt Cb in einer inaktiven Konformation. Die Neuroligine (NL) 2 und 4 können mit der SH3 – Domäne des Cb wechselwirken und Cb in eine aktive Konformation überführen. GlyRs welche aufgrund Ihrer fehlenden Wechselwirkung mit dem postsynaptischen Gephyrin-Collybistin-Komplex nicht interagieren können sind extrasynaptisch in der Zellmembran lokalisiert. Die Untersuchungen mittels der Zwei-Elektroden-Spannungsklemme (engl. TEVC) nach heterologer Expression in den Oozyten des afrikanischen Krallenfroschs Xenopus laevis ergaben, dass die intrazellulär lokalisierten Proteine Gephyrin und Collybistin II SH3-, die apparente Glyzin – Affinität des heteropentameren GlyR α1β und des homopentameren GlyR α1-gbm um das 1,5 bis 10-fache verringern, jedoch keine apparente – Glyzin - Affinitätsveränderung beim homooligomeren GlyR α1-wt bewirken. Nach dem Ausschalten der membranwechselwirkenden PH – Domäne von Collybistin II SH3- durch Punktmutationen in der PH – Domäne (RR363-364-NN) ist keine Verringerung der apparenten Glyzin - Affinität mehr zu beobachten. Neuroligin 2 verringert die apparente Glyzin-Affinität des GlyR α1-gbm nach Ko – Expression mit Gephyrin und der inaktiven Collybistin II SH3+ Variante. Gephyrin und Collybistin II SH3- verringern die apparente GABA – Affinität bei GABAAR α1β3 und GABAAR α2β3. Bei GABAAR α2β3 ist dieser Effekt auch auch ohne die Ko – Expression mit Gephyrin zu beobachten. Es konnte ebenfalls gezeigt werden, dass Gephyrin und Collybistin II SH3- die apparente Glyzin – und Glutamat – Affinität des exzitatorisch fungierenden N-Methyl-D-Aspartat Rezeptors (NMDA), nach der Einklonierung des Gephyrin – Bindemotivs in die NR1 – Untereinheit, verringern. Weiterhin konnte gezeigt werden, dass die Interaktion von Gephyrin und Collybistin II SH3- mit GlyR α1-gbm zu einer drastischen Reduktion des partiellen Agonisten Taurin vermittelten maximal induzierbaren Stroms führt. Die Oligomisierungsdomänen von Gephyrin beeinflussen die Verringerung der apparenten Glyzin - Affinität des GlyR α1-gbm. Es konnte in dieser Arbeit gezeigt werden, dass die Auswirkungen der Gerüstproteine Gephyrin und Collybistin durch eine funktionelle Analyse von ionotropen Rezeptoren mittels TEVC charakterisiert werden können. Somit konnte das Ziel der Etablierung einer zur Validierung geeigneten Methode für die Untersuchung von Rezeptoren mit intrazellulären Proteinen erreicht werden. 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It is as significant as the more established linear and angular acceleration (usually acquired by inertial measurement units). There have been several studies involving planar pressure in the discipline of activity recognition, as reviewed in the first chapter. These studies have shown that planar pressure is a promising sensing modality for activity recognition. However, they still take a niche part in the entire discipline, using ad hoc systems and data analysis methods. Mostly these studies were not followed by further elaborative works. The situation calls for a general framework that can help push planar pressure sensing into the mainstream. This dissertation systematically investigates using planar pressure distribution sensing technology for ubiquitous and wearable activity recognition purposes. We propose a generic Textile Pressure Mapping (TPM) Framework, which encapsulates (1) design knowledge and guidelines, (2) a multi-layered tool including hardware, software and algorithms, and (3) an ensemble of empirical study examples. Through validation with various empirical studies, the unified TPM framework covers the full scope of application recognition, including the ambient, object, and wearable subspaces. The hardware part constructs a general architecture and implementations in the large-scale and mobile directions separately. The software toolkit consists of four heterogeneous tiers: driver, data processing, machine learning, visualization/feedback. The algorithm chapter describes generic data processing techniques and a unified TPM feature set. The TPM framework offers a universal solution for other researchers and developers to evaluate TPM sensing modality in their application scenarios. The significant findings from the empirical studies have shown that TPM is a versatile sensing modality. Specifically, in the ambient subspace, a sports mat or carpet with TPM sensors embedded underneath can distinguish different sports activities or different people's gait based on the dynamic change of body-print; a pressure sensitive tablecloth can detect various dining actions by the force propagated from the cutlery through the plates to the tabletop. In the object subspace, swirl office chairs with TPM sensors under the cover can be used to detect the seater's real-time posture; TPM can be used to detect emotion-related touch interactions for smart objects, toys or robots. In the wearable subspace, TPM sensors can be used to perform pressure-based mechanomyography to detect muscle and body movement; it can also be tailored to cover the surface of a soccer shoe to distinguish different kicking angles and intensities. All the empirical evaluations have resulted in accuracies well-above the chance level of the corresponding number of classes, e.g., the `swirl chair' study has classification accuracy of 79.5% out of 10 posture classes and in the `soccer shoe' study the accuracy is 98.8% among 17 combinations of angle and intensity.\",\"descriptionType\":\"Abstract\"},{\"lang\":null,\"description\":\"Schriftenreihe / Fachbereich Informatik, D386\",\"descriptionType\":\"SeriesInformation\"}],\"geoLocations\":[{\"geoLocationPlace\":\"Kaiserslautern\"}],\"fundingReferences\":[],\"url\":\"https://kluedo.ub.uni-kl.de/5782\",\"contentUrl\":null,\"metadataVersion\":1,\"schemaVersion\":\"http://datacite.org/schema/kernel-4\",\"source\":\"fabricaForm\",\"isActive\":true,\"state\":\"findable\",\"reason\":null,\"landingPage\":{\"url\":\"https://kluedo.ub.uni-kl.de/frontdoor/index/index/docId/5782\",\"error\":\"\",\"status\":200,\"checked\":\"2020-05-01 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Therefore, much effort has been extended toward adding value to biowastes of agri-food industries through biorefinery processing approaches. This study focused, for the first time, on the valorization of tomato by-products of a Tunisian industry for the recovery of value-added compounds using biorefinery cascade processing. Results: The process integrated supercritical CO2 extraction of carotenoids within the oil fractions from tomato seeds (TS) and tomato peels (TP), followed by a batch isolation of protein from the residues. The remaining lignocellulosic matter from both fractions was then submitted to a liquid hot water (LHW) hydrolysis. Supercritical CO2 experiments extracted 5.79% oleoresin, 410.53 mg lycopene/kg, and 31.38 mg β-carotene/kg from TP and 26.29% oil, 27.84 mg lycopene/kg, and 5.25 mg β-carotene/kg from TS, on dry weights. Protein extraction yields, nearing 30% of the initial protein contents equal to 13.28% in TP and 39.26% in TS, revealed that TP and TS are a rich source of essential amino acids. LHW treatment run at 120–200 °C, 50 bar for 30 min showed that a temperature of 160 °C was the most convenient for cellulose and hemicellulose hydrolysis from TP and TS, while keeping the degradation products low. Conclusions: Results indicated that tomato by-products are not only a green source of lycopene-rich oleoresin and tomato seed oil (TSO) and of protein with good nutritional quality but also a source of lignocellulosic matter with potential for bioethanol production. 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Zum Einsatz kommen Strahlmittel, die aus Stahlkernen mit zinkreichen Schalen bestehen. Eine Schicht einer sproeden intermetallischen Zink-Eisen-Phase am Uebergang zwischen Schale und Kern bewirkt ein Abplatzen eines Teils der Schale beim Auftreffen der Strahlmittelteilchen. Die Bruchstuecke werden teilweise oder ganz durch ein Reibschweissen auf die Werkstueckoberflaeche aufgetragen. Modellversuche mit einer druckluftbeaufschlagten manuellen Strahlvorrichtung und Versuche mit industriell eingesetzten mechanischen Strahlanlagen zeigen, dass nur ein Teil der Oberflaeche mit einer Beschichtung bedeckt wird. Proben aus Stahl, Kupfer und Aluminium wurden unter verschiedenen Einfallwinkeln und mit unterschiedlicher kinetischer Energie des Strahlmittels behandelt. Die Bestimmung von Schichtgewicht und Flaechenbedeckung der aufgetragenen Schichten erfolgte durch Einsatz von gravimetrischen und rasterelektronenmikroskopischen Verfahren. Die Flaechenbedeckung erreicht einen Saettigungswert, waehrend das Schichtgewicht linear mit der Strahlzeit zunimmt. Es konnte gezeigt werden, dass ein Verschweissen und damit eine Beschichtung nur stattfindet, wenn die Verformung der Werkstueckoberflaeche lokal ausreicht, um Oxidschichten zu entfernen. Halbempirische Ansaetze beschreiben den zunehmenden Einfluss von Strahlzeit, Strahlwinkel und kinetischer Energie der Strahlmittelteilchen auf die Flaechenbedeckung und das Schichtgewicht. Spiketest und Vorwaertsfliesspressen zur Pruefung des Einflusses der Beschichtungen auf die Umformeignung zeigen, dass die Anlagerung von Festschmiermitteln wie Graphit durch die Topographie des Beschichtungsmaterials verbessert wird. In Verbindung mit Schmiermitteln erzielen mechanisch beschichtete Proben vergleichbare Umformkraefte und Spikehoehen wie die als Referenz dienenden phosphatierten und mit Seife behandelten Proben. Industrielle Umformversuche bestaetigen die Anwendbarkeit des Verfahrens bei verschiedenen Fertigungsarten. 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