sanger/limber

<%# Driver file for Hamilton robot to transfer from LRC PBMC Pools (or Input) to LRC GEM x 5P chip %>
<%= CSV.generate_line [
    'Workflow', 
    @workflow
  ], 
  row_sep: '' 
%>
<%= CSV.generate_line [], row_sep: "" %>
<%= CSV.generate_line [
                        'Source Plate ID',
                        'Source Plate Well',
                        'Destination Plate ID',
                        'Destination Plate Well',
                        'Source Well Volume',
                        'Sample Volume',
                        'PBS Volume',

                      ],
                      row_sep: ""
%>
<%
  # Configuration specific to this export

  required_number_of_cells = 30000 
  wastage_factor = 0.95238 # accounting for wastage of material when transferring between labware
  chip_loading_concentration = 2400 
  desired_number_of_cells_per_chip_well = 90000
  desired_sample_volume = 37.5 # microlitres
  volume_taken_for_cell_counting = 10.0 # microlitres

  # Destination wells are mapped to numbers: A1 -> 17, A2 -> 18, ..., A8 -> 24
  mapping = (1..8).each_with_object({}) { |i, hash| hash["A#{i}"] = (16 + i).to_s }

  # Make a mapping { src_location => src_well  }
  ancestral_plate_wells = @ancestor_plate.wells.index_by(&:location)
  rows_array = []
  each_source_metadata_for_plate(@plate) do |src_barcode, src_location, dest_well|
    number_of_samples =  ancestral_plate_wells[src_location].aliquots.length
    resuspension_volume = (number_of_samples * required_number_of_cells * wastage_factor).to_f / chip_loading_concentration
    source_well_volume = resuspension_volume - volume_taken_for_cell_counting
    sample_volume = desired_number_of_cells_per_chip_well.to_f/chip_loading_concentration 
    pbs_volume = desired_sample_volume - sample_volume
    rows_array << [
      src_barcode,
      src_location,
      @plate.labware_barcode.human,
      mapping[dest_well.location],
       '%0.2f' % source_well_volume,
       '%0.2f' % sample_volume,
       '%0.2f' % pbs_volume
    ]
  end
%>
<% rows_array.each do |row| %>
<%= CSV.generate_line row, row_sep: "" %>
<% end %>