sanger/limber

<%= page(:'pcr-cycles-binned-plate') do -%>
  <%= content do %>
    <%= card title: 'Help' do %>
      <p>Upload the customer completed csv file describing your desired pcr cycles binning strategy. An example is shown below:</p>
      <p>Please make sure there is a header row for the parent plate barcode, this barcode must match the plate from which the dilution plate is being created. Then leave a spacer row before the row column headings.
      <p>Please also make sure you specify source and diluent volumes, number of pcr cycles, and whether the well should be submitted for sequencing (Y/N) for each well that has a sample. If sequencing, you also need to supply Sub-Pool and Coverage values.</p>
      <table class='table table-sm'>
        <thead>
          <tr>
            <th>Plate barcode</th>
            <td>DN12345678A</td>
          </tr>
          <tr>
            <td></td>
          </tr>
          <tr>
            <th>Well</th>
            <th>Concentration (nM)</th>
            <th>Sanger Sample Id</th>
            <th>Supplier Sample Name</th>
            <th>Input amount available (fmol)</th>
            <th>Input amount desired</th>
            <th>Sample volume</th>
            <th>Diluent volume</th>
            <th>PCR cycles</th>
            <th>Submit for sequencing (Y/N)?</th>
            <th>Sub-Pool</th>
            <th>Coverage</th>
          </tr>
        </thead>
        <tbody>
          <tr><td>A1</td><td>15.2</td><td>101</td><td>Smp 1</td><td>45.3</td><td>50.0</td><td>5.0</td><td>20.0</td><td>12</td><td>Y</td><td>1</td><td>250</td></tr>
          <tr><td>B1</td><td>0.12</td><td>102</td><td>Smp 2</td><td>7.1</td><td>50.0</td><td>25.0</td><td>0.0</td><td>16</td><td>N</td><td></td><td></td></tr>
          <tr><td>C1</td><td>8.7</td><td>103</td><td>Smp 3</td><td>30.4</td><td>50.0</td><td>10.0</td><td>15.0</td><td>12</td><td>Y</td><td>1</td><td>250</td></tr>
          <tr><td>D1</td><td>21.3</td><td>104</td><td>Smp 4</td><td>25.0</td><td>50.0</td><td>4.2</td><td>18.8</td><td>12</td><td>Y</td><td>1</td><td>250</td></tr>
          <tr><td>E1</td><td>11.8</td><td>105</td><td>Smp 5</td><td>16.9</td><td>50.0</td><td>8.7</td><td>16.3</td><td>14</td><td>Y</td><td>1</td><td>250</td></tr>
          <tr><td>F1</td><td>9.1</td><td>106</td><td>Smp 6</td><td>36.2</td><td>50.0</td><td>9.6</td><td>17.1</td><td>14</td><td>Y</td><td>2</td><td>250</td></tr>
          <tr><td>G1</td><td>0.03</td><td>107</td><td>Smp 7</td><td>19.7</td><td>50.0</td><td>25.0</td><td>0.0</td><td>12</td><td>N</td><td></td><td></td></tr>
          <tr><td>H1</td><td>1.8</td><td>108</td><td>Smp 8</td><td>16.6</td><td>50.0</td><td>23.5</td><td>1.5</td><td>14</td><td>Y</td><td>2</td><td>250</td></tr>
          <tr><td>A2</td><td>7.6</td><td>109</td><td>Smp 9</td><td>42.2</td><td>50.0</td><td>12.6</td><td>12.4</td><td>16</td><td>Y</td><td>2</td><td>250</td></tr>
          <tr><td>B2</td><td>14.2</td><td>110</td><td>Smp 10</td><td>29.5</td><td>50.0</td><td>9.5</td><td>15.4</td><td>12</td><td>Y</td><td>2</td><td>250</td></tr>
        </tbody>
      </table>
      <p>In this example we will generate 3 bins:</p>
      <ul>
        <li>16 cycles: Source wells B1, A2 into A1 and B1</li>
        <li>14 cycles: Source wells E1, F1, H1 into A2 to C2</li>
        <li>12 cycles: Source wells A1, C1, D1, G1, B2 into A3 to E3</li>
      </ul>
      <p>NB. All wells with aliquots in the parent must have values and be PCR amplified, but not all need to be submitted for sequencing. e.g. B2 and G1 above. These do not need Sub-Pool or Coverage values.</p>
    <% end %>
  <% end %>
  <%= sidebar do %>
    <%= card title: 'File upload' do %>
      <%= form_for(@labware_creator, as: :plate, url: limber_plate_children_path(@labware_creator.parent)) do |f| %>
        <%= f.hidden_field :purpose_uuid %>
        <div class="form-group">
          <%= f.file_field :file, accept: '.csv', required: true %>
        </div>
        <%= f.submit class: 'btn btn-success' %>
      <% end %>
    <% end %>
  <% end %>
<%- end -%>